RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines

Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Bacteria - Gram negative Hemophilus influenzae

Get tips on using EZ-RNA Total RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Brain

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized Caov-3

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized MDAH 2774

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized OVCAR-3

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized SKOV-3

Get tips on using MagExtractor -RNA to perform RNA isolation / purification Cells - immortalized COV-434

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780-CIS

Get tips on using miRcute miRNA Isolation Kit to perform RNA isolation / purification Cells - immortalized H1299

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.