siRNA / miRNA gene silencing Human UCC (urothelial cancer cell lines)

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Get tips on using Gibco™ DMEM, high glucose to perform 3D Cell Culture Media Human cancer esophageal organoids

Products Fisher Scientific Gibco™ DMEM, high glucose

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray Gene expression arrays A-375 human melanoma Digoxigenin-11-dUTP

Get tips on using MethylFlash Hydroxymethylated DNA Quantification Kit to perform DNA methylation profiling Whole genome profiling - human germ cell cancer

Products Epigentek MethylFlash Hydroxymethylated DNA Quantification Kit

Get tips on using SNP Type™ 96.96 Genotyping Reagent Kit with Control Line Fluid—10 IFCs to perform Cell line authentication Human prostatic cancer cell line DU145

Products Fluidigm SNP Type™ 96.96 Genotyping Reagent Kit with Control Line Fluid—10 IFCs

Get tips on using SNP Type™ 96.96 Genotyping Reagent Kit with Control Line Fluid—10 IFCs to perform Cell line authentication Human prostatic cancer cell line PC3

Products Fluidigm SNP Type™ 96.96 Genotyping Reagent Kit with Control Line Fluid—10 IFCs

Get tips on using DeadEnd™ Fluorometric TUNEL System to perform TUNEL assay cell type - HeLa cells human cervical cancer

Products Promega DeadEnd™ Fluorometric TUNEL System

Get tips on using Anti-LC3 antibody produced in rabbit to perform Autophagy assay cell type - Human osteosarcoma cancer cells

Products Sigma-Aldrich Anti-LC3 antibody produced in rabbit

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

Cellular assays Cell cycle assay human AGS cell line

Get tips on using DeadEnd™ Fluorometric TUNEL System to perform TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer

Products Promega DeadEnd™ Fluorometric TUNEL System

Get tips on using ApoBrdU Red DNA Fragmentation Kit to perform TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer

Products Biovision ApoBrdU Red DNA Fragmentation Kit

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