dna-quantification-human-pc-3

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - THP 1

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - Hep G2

Get tips on using QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Human - Deletion PC-3 AGR3

Get tips on using QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Human - Deletion PC-3 AGR2

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - MDA-MB-231

Get tips on using Cell Cycle Assay Kit (Fluorometric - Green) to perform DNA quantification Human - Hep G2

Get tips on using MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging to perform ROS assay cell type - PC-3 human prostate adenocarcinoma

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Human - Colorectal aenocarenoma (SW48) - paraffin embeded