DNA methylation profiling Gene specific profiling UMR-106

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Get tips on using Wizard® Plus Maxipreps DNA Purification System to perform Plasmid Isolation E. coli DH5α

Get tips on using Illustra GFX PCR DNA and Gel Band Purification kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using ARCTURUS® PicoPure® DNA Extraction Kit to perform RNA isolation / purification Cells - primary human melanocytes

Get tips on using Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells to perform Reporter gene assay β-galactosidase substrates - yeast, Yarrowia lipolytica