DNA transfection Mammalian cells Primary cells

Get tips on using miRNeasy Micro Kit to perform RNA isolation / purification Cells - primary rat cortical neurons

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human lung fibroblasts

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human dermal fibroblasts

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary human dermal fibroblasts

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human chondrocytes - osteoarthritis

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human cardiac fibroblasts

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human CD14+ monocytes

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Cells - primary human osteoblasts

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary mouse oocytes

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.