Immunohistochemistry Collagen Type I Goat

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - Raw 264.7

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - 3T3-L1

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - MCF-7

Get tips on using Anti-PI3-kinase p85-α antibody produced in rabbit to perform Autophagy assay cell type - HepG2

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - Islets of langerhans (Beta cells)

Get tips on using 8 µm Chemotaxis Assays, 96-Well Format to perform Cell migration / Invasion cell type - MCF-10A

Get tips on using 8 µm Chemotaxis Assays, 24-Well Format to perform Cell migration / Invasion cell type - PANC-1

Get tips on using Radius™ 24-Well Cell Migration Assay to perform Cell migration / Invasion cell type - SH-SY5Y

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using Leukocyte Alkaline Phosphatase Kit to perform Acid phosphatase assay cell type - human periodontal ligament cells