RNA sequencing Mouse

Get tips on using RNeasy Plus Micro Kit to perform RNA isolation / purification Tissue - Mouse Cerebellum

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Mouse Brain

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Mouse Oviduct

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Mouse Ovarian

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Mouse Hypothalamus

Get tips on using RNeasy Plus Universal Kits to perform RNA isolation / purification Tissue - Mouse Thymus

Get tips on using EZ-RNA Total RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Brain

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Monoclonal Mouse Anti-Human Cytokeratin 7 (Dako Omnis) Clone OV-TL 12/30 to perform Immunohistochemistry Human - CK7