Cell Culture Contamination Detection Kit

Get tips on using MRS BROTH (DE MAN, ROGOSA, SHARPE) to perform Bacterial cell culture media Lactobacillus plantarum

Get tips on using HyClone™ Dulbecco's Modified Eagles Medium to perform Mammalian cell culture media MCF-7

Get tips on using HyClone™ Dulbecco's Modified Eagles Medium to perform Mammalian cell culture media PANC-1

Get tips on using Gibco™Ham's F-12K (Kaighn's) Medium to perform Stem cell culture media hTrophoblasts

Get tips on using MGMTM-4 Melanocyte Growth Medium-4 BulletKitTM to perform Mammalian cell culture media HEM

Get tips on using EpiLife™ Medium, with 60 µM calcium to perform Mammalian cell culture media HEK

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse primary lung epithelial cells-organoids

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse primary mammary ephitelial cells- organoids

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.