DNA isolation / purification Tissue

Get tips on using Gentra Puregene Blood Kit to perform DNA isolation / purification Cells - Immortalized cell lines Lymphoblastoid cell lines

Get tips on using Gentra Puregene Buccal Cell Kit (100) to perform DNA isolation / purification Cells - Primary cells Buccal cells

Get tips on using High Pure PCR Template Preparation Kit to perform DNA isolation / purification Cells - Primary cells Rat astrocytes

Get tips on using FastDNA™ SPIN Kit for Feces to perform DNA isolation / purification Bacteria - Gram positive Clostridium difficile

Get tips on using RNAprotect Tissue Reagent (250 ml) to perform RNA stabilization Sorted cells

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using MinElute PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using Purelink PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using QIAquick PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb