Cell Isolation

Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - MCF-7

Get tips on using BrdU Cell Proliferation Assay to perform Cell cytotoxicity / Proliferation assay cell type - SKOV-3

Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - OVCAR-3

Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - DU-145

Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - PC-3

Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - Hep G2

Get tips on using Cell Proliferation ELISA, BrdU to perform Cell cytotoxicity / Proliferation assay cell type - Hep G2

Get tips on using Cell Proliferation ELISA, BrdU to perform Cell cytotoxicity / Proliferation assay cell type - 3T3-L1

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Contamination can affect cell characteristics, i.e., growth, metabolism, and morphology leading to unreliable and erroneous experimental data. Depending on the source of contaminants, one can detect contamination by using a light microscope, gram stain, isothermal amplification, or PCR. Bacteria and fungi can usually be identified by optical microscopy. Mycoplasma in cell cultures cannot be detected visually. Hence, these microbes can go unnoticed for long periods and are determined using dedicated assays. Early and rapid identification of contaminants is vital to detect, handle and prevent contamination for good cell-culture practices. However, detection and identification can be challenging and tricky based on usual visual identifications. Hence it is essential to use a standard contamination detection kit to detect and maintain best practices.