Site Directed Mutagenesis (SDM) Mouse Deletion 3T3-L1

Get tips on using HSP70 ELISA Kit (ab133060) to perform ELISA Mouse - HSP70

Get tips on using Net siRNA (m) to perform siRNA / miRNA gene silencing Mouse - FL83B Elk3/Net

Get tips on using p130 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - mESC Rbl2/p130

Get tips on using MITF siRNA (m) to perform siRNA / miRNA gene silencing Mouse - B16-F10 MITF

Get tips on using TLR4 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - Neuro 2a TLR4

Get tips on using C23 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - Neuro 2a C23

Get tips on using iBONi siRNA pool to perform siRNA / miRNA gene silencing Mouse - B16-F10 P53

Get tips on using Nrf2 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - B16-F10 Nrf2

Get tips on using Rn_Tlr3_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - Neuro 2a TLR3

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.