rna-isolation-purification-cells-immortalized-ags

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - primary human cardiac fibroblasts

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary mouse dorsal root ganglion neurons

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human peripheral blood monocytes

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human osteoblasts - rheumatoid arthritis

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Brain

Get tips on using E.Z.N.A.® Yeast RNA Kit to perform RNA isolation / purification Yeast - Schizosaccharomyces pombe

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Yeast - Cryptococcus neoformans

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Thymus

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Spleen