shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3)

Get tips on using Lipofectamine® LTX Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa

Get tips on using Lipofectamine® LTX Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines BXPC3

Get tips on using EpiTect Fast 96 Bisulfite Kit to perform Bisulfite DNA Modification Cell lines / primary cells

Get tips on using EpiTect Fast Bisulfite Kit (10) to perform Bisulfite DNA Modification Cell lines / primary cells

Get tips on using EpiTect Plus DNA Bisulfite Kit to perform Bisulfite DNA Modification Cell lines / primary cells

Get tips on using Monoclonal Mouse Anti-Bromodeoxyuridine (Concentrate) Clone Bu20a to perform Immunohistochemistry Mouse - BrdU

Get tips on using siGENOME Rat Nrp1 siRNA to perform siRNA / miRNA gene silencing Rat - Schwann cells Nrp1

Get tips on using SMARTpool: ON-TARGETplus TP63 siRNA to perform siRNA / miRNA gene silencing Human - A253 P36

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.