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Site Directed Mutagenesis (SDM) Rat

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Get tips on using siGENOME Human DAB2 (1601) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A549 DAB2

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An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells RAW 264.7

Get tips on using siGENOME Mouse Sod2 (20656) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - RGC-5 Sod2

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Get tips on using siGENOME Human PDCD1LG2 (80380) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - M244 PD-L2

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Get tips on using siGENOME Human PPARD (5467) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - DU145 PPAR-delta

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Get tips on using siGENOME Human ATG12 (9140) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BT-20 Atg12

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Get tips on using siGENOME Human ATG7 (10533) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - LN-18 ATG-7

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Get tips on using siGENOME Human MAPKAPK2 (9261) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - LN-18 MK2/MAPKAPK2

Products Horizon Discovery Ltd. siGENOME Human MAPKAPK2 (9261) siRNA - SMARTpool

Get tips on using siGENOME Human RIPK3 (11035) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - LN-18 RIP3/RIPK3

Products Horizon Discovery Ltd. siGENOME Human RIPK3 (11035) siRNA - SMARTpool

Get tips on using siGENOME Human AP1G1 (164) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HeLa γ1-adaptin/AP1G1

Products Horizon Discovery Ltd. siGENOME Human AP1G1 (164) siRNA - SMARTpool

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