dna-isolation-purification-bacteria-gram-positive-clostridium-difficile

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Bacteria - Gram negative Haemophilus influenzae

Get tips on using QIAamp DNA Micro Kit to perform DNA isolation / purification Bacteria - Gram negative Helicobacter pylori

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Bacteria - Gram negative Salmonella enterica

Get tips on using MagAttract HMW DNA Kit (48) to perform DNA isolation / purification Bacteria - Gram negative Klebsiella

Get tips on using MagAttract PowerMicrobiome DNA/RNA EP to perform DNA isolation / purification Bacteria - Gram negative Klebsiella

Get tips on using Aquadien™ DNA Extraction Kit to perform DNA isolation / purification Bacteria - Gram negative Legionella

Get tips on using Chromogenic Culture Media to perform Bacterial cell culture media Clostridium difficile

Get tips on using AllPrep Bacterial DNA/RNA/Protein Kit (50) to perform DNA isolation / purification Bacteria - Gram negative Massilia sp

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.