DNA transfection Mammalian cells Primary cells

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Get tips on using Minimum Essential Medium Eagle to perform Mammalian cell culture media HSG cells

Products Sigma-Aldrich Minimum Essential Medium Eagle

Get tips on using Cytotoxicity Detection Kit (LDH) to perform Live / Dead assay mammalian cells - RAW 264.7

Products Sigma-Aldrich Cytotoxicity Detection Kit (LDH)

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - HepG2

Products Thermo Fisher Scientific RIPA Lysis and Extraction Buffer

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - HEK293T

Products Thermo Fisher Scientific RIPA Lysis and Extraction Buffer

Get tips on using QuantiPro™ BCA Assay Kit to perform Protein quantification Mammalian cells - L929

Products Sigma-Aldrich QuantiPro™ BCA Assay Kit

Get tips on using QuantiPro™ BCA Assay Kit to perform Protein quantification Mammalian cells - RAW264.7

Products Sigma-Aldrich QuantiPro™ BCA Assay Kit

Get tips on using Qubit™ Protein Assay Kit to perform Protein quantification Mammalian cells - HeLa

Products Thermo Fisher Scientific Qubit™ Protein Assay Kit

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase HEK 293 human embryonic kidney cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase BHK-21 baby hamster kidney cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates HeLa cervical cancer cells

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