The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.
DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.
Get tips on using Fluorescent 1 kb DNA Ladder to perform DNA Ladder 1 kb
Get tips on using 1 kb Plus DNA Ladder to perform DNA Ladder 1 kb
Get tips on using Mouse KIM-1 ELISA Kit to perform ELISA Mouse - KIM-1
Get tips on using Endothelin-1 Quantikine ELISA Kit to perform ELISA Mouse - Endothelin 1
Get tips on using Endothelin 1 ELISA Kit (ab133030) to perform ELISA Mouse - Endothelin 1
Get tips on using Mouse Dkk-1 ELISA Kit to perform ELISA Mouse - Dkk-1
Get tips on using HO-1 (rat), ELISA kit to perform ELISA Rat - HO-1
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment