Protein expression and purification Bacteria Escherichia coli

Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Mammalian cells - OUMS-27

Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Yeast - Scheffersomyces (Pichia) stipitis

Get tips on using Tissue or Cell Total Protein Extraction Kit to perform Protein isolation Tissue - Lamb muscle tissue

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Tissue - Mouse prostate tissue

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Tissue - Mouse liver tissue

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Tissue - Mouse lung tissue

Get tips on using M-PER™ Mammalian Protein Extraction Reagent to perform Protein isolation Mammalian cells - HLE-B3

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using pLEXSY-hyg-2-hAm to perform Protein Expression Eukaryotic cells - Iranian lizard Leishmania cells recombinant human amelogenin