rna-isolation-purification-cells-immortalized-saos-2

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - mouse brain tissue

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - mouse adipose tissue

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - mouse liver tissue

Get tips on using QIAamp Viral RNA Mini Kit (50) to perform RNA isolation / purification Viral - Hazara virus

Get tips on using AllPrep DNA/RNA/miRNA Universal Kit to perform RNA isolation / purification Tissue - Mouse Adipose

Get tips on using AllPrep DNA/RNA/miRNA Universal Kit to perform RNA isolation / purification Tissue - Human Lung

Get tips on using Aurum™ Total RNA Mini Kit to perform RNA isolation / purification Tissue - Human Subcutaneous

Get tips on using RiboMinus™ Transcriptome Isolation Kit, bacteria to perform RNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Tissue - Human Salivary glands