Cell line authentication

Get tips on using Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit to perform Necrosis A549

Get tips on using Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse to perform Western blotting PCNA

Get tips on using Corning® BioCoat™ Angiogenesis System: Endothelial Cell Tube Formation to perform Angiogenesis assay human - HMVEC

Get tips on using Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit to perform Angiogenesis assay human - HMVEC

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide (PI) to perform Apoptosis assay cell type - HeLa cells

Get tips on using LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation to perform Live / Dead assay mammalian cells - rat testicular tissue

Get tips on using Host Cell Residual DNA contamination LANCE Ultra TR-FRET Detection Kit, 500 Assay Points to perform Cell Culture Contamination Detection Kit Bacteria