RNA isolation / purification Cells Cancer cell lines

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Thyroid gland

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Spinal cord

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Get tips on using Maxwell® 16 LEV simplyRNA Purification Kit to perform RNA isolation / purification Tissue - Human Muscles

Get tips on using Absolutely RNA FFPE Kit to perform RNA isolation / purification Tissue - Human Kidney

Get tips on using NucleoSpin® RNA Plus to perform RNA isolation / purification Tissue - Mouse Retina

Get tips on using Total RNA Kit, peqGOLD to perform RNA isolation / purification Tissue - Human Stomach

Get tips on using NucleoSpin® RNA XS to perform RNA isolation / purification Tissue - Human Skin

Get tips on using PAXgene Tissue RNA Kit to perform RNA isolation / purification Tissue - Human Colon

Get tips on using NucleoSpin® RNA/Protein to perform RNA isolation / purification Tissue - Human Cornea