Microarray Gene expression arrays Mouse brain tissue

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Small intestine

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Fallopian tubes

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Bone marrow

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Adrenal glands

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - Mouse liver tissue

Get tips on using AllPrep DNA/RNA/miRNA Universal Kit to perform RNA isolation / purification Tissue - Mouse Adipose

Get tips on using RNAqueous™ Total RNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Uterus

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse skeletal muscle