Protein quantification Yeast

Get tips on using MasterPure™ Yeast RNA Purification Kit to perform RNA isolation / purification Yeast - Candida albicans

Get tips on using RiboPure™ RNA Purification Kit, yeast to perform RNA isolation / purification Yeast - Candida albicans

Get tips on using RiboPure™ RNA Purification Kit, yeast to perform RNA isolation / purification Yeast - Ashbya gossypii

Get tips on using RiboPure™ RNA Purification Kit, yeast to perform RNA isolation / purification Yeast - Saccharomyces cerevisiae

Get tips on using Gentra Puregene Yeast/Bact. Kit to perform DNA isolation / purification Bacteria - Gram positive Pseudomonas

The formation of DNA from an RNA template using reverse transcription leads to the formation of double-stranded complementary DNA or cDNA. The challenges with this process include 1. Maintaining the integrity of RNA, 2. Hairpin loops or other secondary structures formed by single-stranded RNA can also affect cDNA synthesis, and 3. DNA-RNA hybrids, which may result when the first strand of cDNA is formed. For the first challenge, using workflows that involve proper isolation and storage of RNA, and maintaining a nuclease-free environment helps obtain RNA with ideal 260/230 ratios. Using a reverse transcriptase that can tolerate high temperatures (50-55oC), overcomes obstacles imposed by secondary RNA structures. Finally, RNase H has the ability to hydrolyze RNA before the formation of a second cDNA strand. It is important to ensure that RNase H activity is optimal because higher RNase H activity leads to premature degradation of the RNA template. Many reverse transcriptases offer built-in RNase H activity.

Get tips on using E.Z.N.A.®Yeast Plasmid Mini Kit to perform Plasmid Isolation S. cerevisiae

Get tips on using 2-D Quant Kit to perform Protein quantification Colorimetric method

Get tips on using RPM Yeast Plasmid Isolation Kit, 100 preps to perform Plasmid Isolation S. cerevisiae

Get tips on using 2-D Quant Kit to perform Protein quantification Mammalian cells - SiHa