RNA isolation / purification Cells Cancer cell lines

Get tips on using PAXgene Tissue RNA/miRNA Kit to perform RNA isolation / purification Tissue - Rat Heart

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Rat Gut

Get tips on using PAXgene Tissue RNA/miRNA Kit to perform RNA isolation / purification Tissue - Rat Brain

Get tips on using EasySep™ Human B Cell Isolation Kit to perform Cell Isolation B cell

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized SKBR3, MDA-MB231 and MCF7

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Bacteria - Gram negative Bordetella pertussis

Get tips on using Naive B Cell Isolation Kit II, human to perform Cell Isolation Naive B cell

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Get tips on using Naive Pan T Cell Isolation Kit, human to perform Cell Isolation Naive Pan T cell

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.