siRNA / miRNA gene silencing Rat INS-1

Get tips on using Mouse KIM1 ELISA Kit (TIM-1) (ab119596) to perform ELISA Mouse - KIM-1

Get tips on using Mouse Heme Oxygenase 1 ELISA Kit (ab204524) to perform ELISA Mouse - HO-1

Get tips on using Human Notch-1 Intracellular Domain Antibody to perform Western blotting Notch1

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary human airway epithelial cells

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary human aortic endothelial cells

Get tips on using Mouse TGF beta 1 ELISA Kit (ab119557) to perform ELISA Mouse - TGF-beta 1

Get tips on using Mouse IL-1 beta ELISA Kit (ab100704) to perform ELISA Mouse - IL-1 beta

Get tips on using Mouse ICAM-1/CD54 Quantikine ELISA Kit to perform ELISA Mouse - ICAM-1/CD54