Protein Expression Prokaryotic cells M. smegmatis

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Autophagy assay cell type - Human tracheobronchial epithelial cells (hTEC) Experiment

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type Human tracheobronchial epithelial cells (hTEC)

Dynabeads™ Untouched™ Human T Cells Kit Product

Get tips on using Dynabeads™ Untouched™ Human T Cells Kit to perform Cell Isolation Human T cells

Products Thermo Fisher Scientific Dynabeads™ Untouched™ Human T Cells Kit

RNA isolation / purification Cells - primary mouse dorsal root ganglion neurons Experiment

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary mouse dorsal root ganglion neurons

LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells Product

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - MC3T3