Microarray Gene expression arrays A-375 human melanoma

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

Get tips on using Arp2 siRNA (h) to perform siRNA / miRNA gene silencing Human - T47-D Arp-2

Get tips on using AMPKα1/2 siRNA (h) to perform siRNA / miRNA gene silencing Human - HepG2 AMPKα1/α2

Get tips on using AMPKα1/2 siRNA (h) to perform siRNA / miRNA gene silencing Human - HepG2 AMPKα1/α2

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using AllStars Hs Cell Death siRNA to perform siRNA / miRNA gene silencing Human - U2OS KRAS

Get tips on using psiCHECK-2vector to perform Reporter gene assay luciferase - HEK 293 human embryonic kidney cells

Get tips on using Notch 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - A2780 Notch 1

Get tips on using CREB-2 siRNA (h) to perform siRNA / miRNA gene silencing Human - HUVEC ATF4 Lipid

Get tips on using pSilencer™ 4.1-CMV neo to perform shRNA gene silencing Human - SiHa AEG-1