rna-isolation-purification-cells-primary-mouse-dorsal-root-ganglion-neurons

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Human Intestine

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Human Heart

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Human Gut

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Tissue - Human Bronchi

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat

Get tips on using PureLink ™ miRNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that no responses other than those related to the signaling pathway of interest. This can be achieved by selecting a highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzyme such as luciferase.

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using MEDOX-Easy Total RNA Extraction Reagent to perform RNA isolation / purification Tissue - Human Mouth