rna-isolation-purification-cells-primary-rat-cortical-neurons

Get tips on using InviMag Universal RNA Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus

Get tips on using ReliaPrep™ RNA Miniprep Systems to perform RNA isolation / purification Bacteria - Gram positive Clostridum botulinum

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Bacillus anthracis

Get tips on using ISOLATE II RNA Micro Kit to perform RNA isolation / purification Bacteria - Gram negative Vibro cholerae

Get tips on using Anti-Estrogen Receptor (ER) (SP1), Rabbit Monoclonal Primary Antibody to perform Immunohistochemistry Estrogen receptor (ER) - Rabbit Human -NA-

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using EZ1 RNA Tissue Mini Kit (48) to perform RNA isolation / purification Tissue - Human FFPE tissue

Get tips on using InviTrap Spin Tissue RNA Mini Kit to perform RNA isolation / purification Tissue - Human Artery / Aorta