rna-isolation-purification-cells-immortalized-bxpc-3

Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform RNA isolation / purification Tissue - human kidney tissue

Get tips on using IncuCyte® Caspase-3/7 Apoptosis Assay Reagent to perform Apoptosis assay cell type - Caspase 3/7

Get tips on using Oct-3/4 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - P19 Oct4

Get tips on using MMP-3 (D7F5B) Rabbit mAb #14351 to perform Immunohistochemistry Rat - MMP3

Get tips on using MagAttract PowerMicrobiome DNA/RNA EP to perform DNA isolation / purification Bacteria - Gram negative Klebsiella

Get tips on using Human Chitinase 3-like 1 DuoSet ELISA to perform ELISA Human - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

Get tips on using JAK1 Antibody (B-3): sc-376996 to perform Western blotting Jak1

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.