siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells

Get tips on using EpiTect Fast LyseAll Bisulfite Kit (200) to perform Bisulfite DNA Modification Cell lines / primary cells

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary mouse dorsal root ganglion neurons

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat dorsal root ganglion neurons

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - primary mouse cortical neurons

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Cells - primary rat cortical neurons

Get tips on using Absolutely Total RNA Purification Kits to perform RNA isolation / purification Cells - primary rat cardiac fibroblasts

Get tips on using CellTiter-Glo® Luminescent Cell Viability Assay to perform Cell cytotoxicity / Proliferation assay cell type - BxPc-3 human primary pancreatic adenocarcinoma

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform Microarray Gene expression arrays - Rhesus monkey brain tissue Biotin

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform 3D Cell Culture Media Primary human breast tumors-Mammospheres

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.