Stem cell Differentiation media

Get tips on using REPLI-g Cell WGA & WTA Kit (48) to perform Whole Transcriptome Amplification Virus

Get tips on using REPLI-g WTA Single Cell Kit (96) to perform Whole Transcriptome Amplification Virus

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay-Silver to perform DNA Damage Assay HCT 116

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay-Silver to perform DNA Damage Assay DLD-1

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay-Silver to perform DNA Damage Assay Caco-2

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Oris™ Universal Cell Migration Assembly Kit, 96 wells to perform Wound healing assay cell type - human MCF-7

Get tips on using Tissue or Cell Total Protein Extraction Kit to perform Protein isolation Tissue - Lamb muscle tissue

Get tips on using BacTiter-Glo™ Microbial Cell Viability Assay to perform Live / Dead assay yeast - Saccharomyces cerevisiae

Get tips on using BacTiter-Glo™ Microbial Cell Viability Assay to perform Live / Dead assay bacteria - Staphylococcus epidermidis