siRNA / miRNA gene silencing Rat Brain endothelial cells HIF-1α

Get tips on using Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System to perform Reporter gene assay β-galactosidase substrates - U87 and U251 glioblastoma cells

Get tips on using MessageAmp II aRNA Amplification Kit to perform Microarray RNA amplification & Labeling - Rhesus monkey brain tissue Biotin

Get tips on using Cy3 Mono-Reactive Dye Pack to perform Microarray RNA amplification & Labeling - Human brain tissue Cyanine 3

Get tips on using MessageAmp II aRNA Amplification Kit to perform Microarray RNA amplification & Labeling - Human brain tissue Cyanine 3

Get tips on using RediPlate™ 96 RiboGreen™ RNA Quantitation Kit to perform RNA quantification Fuorimetric - human brain tissue

Get tips on using Viromer® RED to perform DNA transfection Mammalian cells - Primary cells Rat schwann cells

Get tips on using miRNeasy Micro Kit to perform RNA isolation / purification Cells - primary rat cortical neurons

Get tips on using HTRA2 MISSION shRNA Lentiviral Transduction Particles HtrA serine peptidase 2 to perform shRNA gene silencing Mouse - FL83B HtrA2

Get tips on using STEMdiff™ Cerebral Organoid Maturation Kit to perform Stem cell culture media Brain organoids from Human iPSCs

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.