CRISPR Rat Deletion INS-1 832/13

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Ovarian cancer cell line PA-1

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Renal carcinoma cell line Caki-1

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Colon cancer cell line DLD-1

Get tips on using ON-TARGETplus Human BSG / emmprin (682) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BCP-1 Emmprin / BSG

Get tips on using Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - PANC-1

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm to perform Cell migration / Invasion cell type - SK-MEL-1

Get tips on using MCDB 131 Medium to perform Mammalian cell culture media PCAEC

Get tips on using MCDB 131 Medium to perform Mammalian cell culture media CADMEC/HMVEC

Get tips on using GeneArt™ Site-Directed Mutagenesis System to perform Site Directed Mutagenesis (SDM) Mouse - Point mutation 3T3-L1 S6 kinase 1