ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.
Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - HepG2
Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - HEK293T
Isolating RNA from tissues and paraffin embeded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the intigrity of RNA
Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - Rat_Mesenteric fat
Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - BHK-21
Get tips on using GeneChip™ Human Genome U133A 2.0 Array to perform Microarray Comperative genomic hybridization - Human Tumor
Get tips on using ProcartaPlex Human Cytokine Panel 1B, 25 plex to perform ELISA (kit) Human Serum Cytokine measurements (Multiplex assay) - -NA- Human -NA-
Get tips on using MammoCult™ Human Medium Kit to perform 3D Cell Culture Media Primary human breast tumors-Mammospheres
Get tips on using illustra tissue and cells genomicPrep Mini Spin Kit to perform DNA isolation / purification Tissue - kidney
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