Protein expression and purification Tissue

Get tips on using PRO-PREP™ Protein Extraction Solution (C/T) to perform Protein isolation Mammalian cells - 3T3-L1

Get tips on using Quick Start™ Bradford Protein Assay Kit 1 to perform Protein quantification Mammalian cells - BV-2

Get tips on using Rat Bone Morphogenetic Protein 2 ELISA to perform ELISA Rat - BMP-2

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Adrenal glands

Get tips on using PRO-PREP™ Protein Extraction Solution (C/T) to perform Protein isolation Mammalian cells - Mouse Epididymal fat

Get tips on using AllPrep DNA/RNA/miRNA Universal Kit to perform RNA isolation / purification Tissue - Mouse Adipose

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using Anti-Prosurfactant Protein C (proSP-C) Antibody to perform Flow cytometry Anti-bodies Mouse - proSP-C

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.