DNA methylation profiling Gene specific profiling Ca Ski

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Get tips on using YAP siRNA (h) to perform siRNA / miRNA gene silencing Human - OV2008 Yap Gene Lipofectamine

Products Santa Cruz Biotechnology YAP siRNA (h)

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Cellular assays TUNEL assay cell type HNSCC Detroit 562 human head and neck tumor cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Cellular assays TUNEL assay cell type A127, U87MG, U251MG, T98G human glioblastoma cells
siRNA SKIL Product

Get tips on using siRNA SKIL to perform siRNA / miRNA gene silencing Human - RWPE SKIL

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siRNA SKIL Product

Get tips on using siRNA SKIL to perform siRNA / miRNA gene silencing Human - LNCap SKIL

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siRNA SKIL Product

Get tips on using siRNA SKIL to perform siRNA / miRNA gene silencing Human - DuCaP SKIL

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Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Proteins Protein isolation Tissue Mouse skeletal muscle
siRNA SKIL Product

Get tips on using siRNA SKIL to perform siRNA / miRNA gene silencing Human - LuCaP-77 SKIL

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siRNA SKIL Product

Get tips on using siRNA SKIL to perform siRNA / miRNA gene silencing Human - PC-3 SKIL

Products Thermo Fisher Scientific siRNA SKIL
siRNA SKIL Product

Get tips on using siRNA SKIL to perform siRNA / miRNA gene silencing Human - MCF-7 SKIL

Products Thermo Fisher Scientific siRNA SKIL

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