siRNA / miRNA gene silencing Rat NPC

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - SKOV3 AGR2

Get tips on using pGL3-Basic Vector to perform Reporter gene assay luciferase - human embryonic stem cells

Get tips on using Senescence Detection Kit - Biovision to perform Reporter gene assay β-galactosidase substrates - H9C2

Get tips on using X-Gal Staining Kit to perform Reporter gene assay β-galactosidase substrates - C2C12

Get tips on using Senescence Detection Kit - Biovision to perform Reporter gene assay β-galactosidase substrates - Huh7

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - mouse pancreatic stellate cells

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - mouse mesenchymal stem cells

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Gene expression arrays - Mouse brain tissue Biotin

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Gene expression arrays - Mouse dorsal skin Biotin

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.