Protein expression and purification Mammalian cells HEK 293

Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.

Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media 293T

Get tips on using Dulbecco’s Modified Eagle’s Medium (DMEM) to perform Mammalian cell culture media 293T

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Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Bacteria - Gram negative Haemophilus influenzae

Get tips on using Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram negative Salmonella enterica

Get tips on using Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram positive Staphylococcus aureus

Get tips on using FuGENE® HD Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells HUVEC