PCR Preamplification of cDNA

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Get tips on using Biotools DNA Polymerase to perform PCR Conventional / Qualitative PCR - bacterial DNA

Products Biotools Biotools DNA Polymerase

Get tips on using EpiTect MSP Kit to perform PCR Methylation specific PCR - Mammalian DNA

Products Qiagen EpiTect MSP Kit

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Apoptosis assay cell type Array of apoptotic proteins

Get tips on using TaqMan® MicroRNA Reverse Transcription Kit to perform cDNA synthesis Cell lines

Products Thermo Fisher Scientific TaqMan® MicroRNA Reverse Transcription Kit

Get tips on using EvaGreen® Dye to perform PCR Quantitative real-time PCR - Bacterial DNA

Products Biotium EvaGreen® Dye

Get tips on using Mastermix 16S Complete to perform PCR Quantitative real-time PCR - Bacterial DNA

Products Molzym Mastermix 16S Complete

Get tips on using HotStarTaq DNA Polymerase (25000) to perform PCR Hot start PCR - Bacterial DNA

Products Qiagen HotStarTaq DNA Polymerase (25000)

Get tips on using QuantiTect Virus Kit (1000) to perform PCR Quantitative real-time PCR - Viral

Products Qiagen QuantiTect Virus Kit (1000)

Get tips on using HotStarTaq Plus DNA Polymerase to perform PCR Hot start PCR - Bacterial DNA

Products Qiagen HotStarTaq Plus DNA Polymerase

Get tips on using GoTaq® DNA Polymerase to perform PCR Conventional / Qualitative PCR - bacterial DNA

Products Promega GoTaq® DNA Polymerase

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