crispr-mouse-activation-3t3-l1-c-ebp

Get tips on using Cell Proliferation ELISA, BrdU to perform Cell cytotoxicity / Proliferation assay cell type - 3T3-L1

Get tips on using CM-H2DCFDA (General Oxidative Stress Indicator) to perform ROS assay cell type - 3T3-L1

Get tips on using lenti dCAS-VP64_Blast to perform CRISPR Human - Activation interferon-γ promoter

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Activation RANKL

Get tips on using Anti-Prosurfactant Protein C (proSP-C) Antibody to perform Flow cytometry Anti-bodies Mouse - proSP-C

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Activation PAX5

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using MTT Cell Viability Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - 3T3-L1

Get tips on using Xfect™ Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines 3T3-L1

Get tips on using GeneJuice® Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines 3T3-L1