Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human hair follicle dermal papilla cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary bovine pulmonary artery smooth muscle cells
Get tips on using Alexa Fluor® 700 Mouse Anti-Mouse NK1.1 to perform Flow cytometry Anti-bodies Mouse - NK1.1
Get tips on using RNeasy Plus Micro Kit to perform RNA isolation / purification Cells - primary human lung microvascular endothelial cells
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human carotid artery endothelial cells
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized SKBR3, MDA-MB231 and MCF7
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using RNeasy Protect Cell Mini Kit (50) to perform RNA isolation / purification Cells - primary human mononuclear cells
Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Primary cells Rat astrocytes
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment