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Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - Cancer cell lines Leukemia cancer cell lines THP-1

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized HeLa, CaSki and C33A (Cervical cancer cells)

Get tips on using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents to perform Protein isolation Tissue - ME epithelial tissue

Get tips on using MICROBExpress™ Bacterial mRNA Enrichment Kit to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus

Get tips on using MICROBExpress™ Bacterial mRNA Enrichment Kit to perform RNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

Get tips on using QIAamp 96 Virus QIAcube HT Kit (5) to perform RNA isolation / purification Viral - SARS-CoV-2

Get tips on using ARCTURUS® PicoPure® DNA Extraction Kit to perform RNA isolation / purification Cells - primary human melanocytes

Get tips on using TruSeq Stranded Total RNA to perform RNA sequencing Mouse - 3T3-L1

Get tips on using Qproteome Mammalian Protein Prep Kit to perform Protein isolation Tissue - Human tissue C-MFPE samples

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.