DNA methylation profiling

Get tips on using pAC2-dual-dCas9VP48-sgExpression to perform CRISPR Human - Activation IL1RN

Get tips on using pAC2-dual-dCas9VP48-sgExpression to perform CRISPR Human - Activation SOX2

Get tips on using pAC154-dual-dCas9VP160-sgExpression to perform CRISPR Human - Activation HIV-1 5′ LTR

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - C6

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - MRC5

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - HT1080

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - L929

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - U87MG

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - MG-63

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.