Site Directed Mutagenesis (SDM) Human Deletion HEK 293T

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using SASI_Hs01_00024301 to perform siRNA / miRNA gene silencing Human - MOLT4 RAG1

Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - LLC PKN3

Get tips on using NM_001350 to perform siRNA / miRNA gene silencing Human - U2OS DAXX

Get tips on using SMARTpool: ON-TARGETplus TP63 siRNA to perform siRNA / miRNA gene silencing Human - A253 P36

Get tips on using Cellstain-Double Staining Kit to perform Live / Dead assay mammalian cells - HepaRG human hepatoma

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Human Heart

Get tips on using Cell Cycle Assay Kit (Fluorometric - Green) to perform DNA quantification Human - Hep G2

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - HeLa

Get tips on using EZ‐ChIP™ Assay Kit (Cat#17–371) to perform ChIP Human - HeLa