shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3) CD24

Cell cytotoxicity assays measure the ability of certain compounds or chemical mediators to reduce the viability of the cells. The term cell cytotoxicity assay can sometimes be used interchangeably with cell proliferation assay. Healthy living cells can be identified by the use of formazan dyes, protease biomarkers or by measuring ATP content. The formazan dyes are chromogenic products formed by the reduction of tetrazolium salts by dehydrogenases, such as lactate dehydrogenase (LDH) and reductases that are released during cell death. Common tetrazolium salts include INT, MTT, MTS and XTT. Cell cytotoxicity can also be measured by using the SRB and WST-1 assays. These assays can usually be used in a high-throughput fashion and can be quantitated by measuring absorbance, colorimetry or luminescence. All these assays require similar numbers of cell plating at the initiation, a time course of treatment with the cytotoxic agent and at least triplicates for each condition at every point of analysis. Cell shrinkage, plasma membrane blebbing, cell detachment, externalization of phosphatidylserine, nuclear condensation and ultimately DNA fragmentation are well-described features of apoptosis. The assays that rely on cell membrane integrity for their function, may not be able to quantify early apoptosis. Therefore, in order to distinguish early apoptotic vs. late apoptotic or necrotic cells, additional flow cytometry techniques can be used. A combination of Annexin V and PI (propidium iodide) can be used to distinguish early (Annexin V+/PI-) and late apoptotic (Annexin V+/PI+) cells. Sometimes, caspase assays are used in order to differentiate the stages of apoptosis.

Get tips on using X-VIVOTM 10 Serum-free Hematopoietic Cell Medium to perform 3D Cell Culture Media Mouse thymic organoids

Get tips on using In Situ Cell Death Detection Kit, TMR red to perform TUNEL assay cell type - Mouse skeletal muscle cells

Get tips on using GenomONE™-Neo HVJ-E Membrane Fusion Transfection Kit to perform siRNA / miRNA gene silencing Human - U937 MK2 (MAPK Kinase 2) Viral vectors

Get tips on using GenomONE™-Neo HVJ-E Membrane Fusion Transfection Kit to perform siRNA / miRNA gene silencing Human - Jurkat MK2 (MAPK Kinase 2) Viral vectors

Get tips on using PE-Cy™7 Mouse Anti-Human CD123 to perform Flow cytometry Anti-bodies Human - CD123/IL3-R

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Whole genome profiling - OVCAR-3 human ovarian cancer

Get tips on using Global DNA Methylation ELISA to perform DNA methylation profiling Whole genome profiling - HCT116, HTC15 human colon cancer cells

Get tips on using Monoclonal Anti-Collagen, Type III antibody produced in mouse to perform Western blotting Type III collagen

Get tips on using NAPSIN A (TMU-AD02) ANTI-HUMAN MOUSE IGG MOAB to perform Immunohistochemistry Human - Naspsin A