Immunohistochemistry Collagen Type VII Rabbit Human

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siRNA / miRNA gene silencing Human - Primary Endometrial Stromal Cells hsa-miR-542-3p Lipid Experiment

RNA siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells hsa-miR-542-3p Lipid

DNA methylation profiling Whole genome profiling - MCF-7, MDA-MB-453 human breast cancer Experiment

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Whole genome profiling MCF-7, MDA-MB-453 human breast cancer

Site Directed Mutagenesis (SDM) Human - Point mutation LNCaP Androgen Receptor splice variant (AR-V) Experiment

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

DNA Site Directed Mutagenesis (SDM) Human Point mutation LNCaP Androgen Receptor splice variant (AR-V)

siRNA / miRNA gene silencing Human - Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1) Lipid Experiment

RNA siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1) Lipid

Dansylcadaverine Product

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - C6