Microarray Gene expression arrays Rat chorid plexus

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Get tips on using BDNF Rat ELISA Kit to perform ELISA Rat - BDNF

Products Thermo Fisher Scientific BDNF Rat ELISA Kit

Get tips on using Rat Activin-A ELISA to perform ELISA Rat - Activin

Products Raybiotech Rat Activin-A ELISA

Get tips on using Rat Adiponectin ELISA Kit to perform ELISA Rat - Adiponectin

Products Sigma-Aldrich Rat Adiponectin ELISA Kit

Get tips on using Tf (Rat) ELISA Kit to perform ELISA Rat - Transferrin (Tf)

Products Abnova Tf (Rat) ELISA Kit

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

DNA Site Directed Mutagenesis (SDM) Rat Point mutation Rat-2 PIK3CB

Get tips on using pcDNA™3.1 (+) Mammalian Expression Vector to perform siRNA / miRNA gene silencing Human - U251 cofilin-1 (CFL1)

Products Thermo Fisher Scientific pcDNA™3.1 (+) Mammalian Expression Vector

Get tips on using β-Galactosidase Reporter Gene Staining Kit to perform Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs)

Products Sigma-Aldrich β-Galactosidase Reporter Gene Staining Kit

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Rabbit eye retina/choroids

Products Sigma-Aldrich CelLytic™ MT Cell Lysis Reagent

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates RAW 264.7

Get tips on using Rat VEGF ELISA Kit (ab100786) to perform ELISA Rat - VEGF

Products Abcam Rat VEGF ELISA Kit (ab100786)

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