DNA transfection Mammalian cells

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Get tips on using BEBMTM Bronchial Epithelial Cell Growth Basal Medium to perform Mammalian cell culture media HBEpC

Products Lonza BEBMTM Bronchial Epithelial Cell Growth Basal Medium

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

RNA siRNA / miRNA gene silencing Human Aortic smooth muscle cell TSP-2

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

RNA siRNA / miRNA gene silencing Human PC3 (human prostate cancer cell line) STEAP2

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

RNA siRNA / miRNA gene silencing Human PC3 (human prostate cancer cell line) HSPA5 (GRP78)

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

RNA siRNA / miRNA gene silencing Human PC3 (human prostate cancer cell line) SIRT1

Get tips on using Viability/Cytotoxicity Assay kit for Bacteria Live and Dead Cells to perform Live / Dead assay bacteria - Salmonella enterica

Products Biotium Viability/Cytotoxicity Assay kit for Bacteria Live and Dead Cells

Get tips on using Gibco™ DMEM, high glucose to perform Mammalian cell culture media MDA-MB-231

Products Fisher Scientific Gibco™ DMEM, high glucose

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media MDA-MB-468

Products Thermo Fisher Scientific DMEM–Dulbecco's Modified Eagle Medium

Get tips on using HyClone™ Dulbecco's Modified Eagles Medium to perform Mammalian cell culture media MCF-7

Products Cytiva HyClone™ Dulbecco's Modified Eagles Medium

Get tips on using HyClone™ Dulbecco's Modified Eagles Medium to perform Mammalian cell culture media PANC-1

Products Cytiva HyClone™ Dulbecco's Modified Eagles Medium

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