siRNA / miRNA gene silencing Human PC-3

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Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized BxPC-3

Products Macherey Nagel NucleoSpin® RNA

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized BxPC-3

Products Qiagen RNeasy Mini Kit

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized SKOV-3

Products Qiagen RNeasy Mini Kit

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Caov-3

Products Qiagen RNeasy Mini Kit

Get tips on using Cell Death Detection ELISAPLUS to perform Apoptosis assay cell type - CaOV-3

Products Sigma-Aldrich Cell Death Detection ELISAPLUS

Get tips on using Annexin V-FITC Kit to perform Apoptosis assay cell type - OVCAR-3

Products Miltenyibiotec Annexin V-FITC Kit

Cellular assays Cell line authentication Human iPSC cells derived from peripheral blood mononuclear cells

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells MDA-MB-231 human breast cancer cells
pGB2B3 Product

Get tips on using pGB2B3 to perform Protein Expression Prokaryotic cells - E. coli hβ-defensin 2/3

Products Rong Gao, Key Laboratory of Bio-Resource and Eco-Environment, Mi pGB2B3

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized Caov-3

Products Qiagen RNeasy Plus Mini Kit

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